The reported case of a desulfovibrio desulfuricans bacteremia occurring in an immunocompromised host with ulcerative colitis confirms that this organism may be a possible. Desulfovibrio desulfuricans strain g20, which was found at an oil well corrosion site 2can. Biocides are frequently used to control sulfatereducing bacteria srb in biofouling. Beyerinck reported the isolation of spirillum desulfuricans from a dutch city canal in delft 6. Get a printable copy pdf file of the complete article 1. The present study addresses the effects of oxygen exposure on the aerobic and anaerobic respiratory activity of desulfovibrio desulfuricans strain dvo1. The complete sequences of the dsra and dsrb genes coding for the and subunits, respectively, of the sulphite reductase enzyme in desulfovibrio desulfuricans were determined. This inherent nadhoxidizingcapacityofthecell free extracts was removed when the phwas adjusted to 5. Sewage contamination caused evolution of large amounts of hydrogen sulfide from. The reported case of a desulfovibrio desulfuricans bacteremia occurring in an immunocompromised host with ulcerative colitis confirms that this organism may be a possible opportunistic human.
Jan 15, 2020 desulfovibrio desulfuricans strain atcc 27774 dsm 6949 is a sulfatereducing bacterium isolated from the rumen of a sheep. Extracellular ironsulfur precipitates from growth of. Pdf this article aims to study hydrogen productionconsumption in desulfovibrio d. Purification and properties of lalanine dehydrogenase. Dde i from desulfovibrio desulfuricans, strain norway sigma.
Coordinated response of the desulfovibrio desulfuricans 27774. This strain was isolated from the highest sulfatereduction positive mostprobablenumber dilution 10 6 of an activated sludge sample, which had been subjected to 120 h of continuous aeration. Since these bacteria are killed by exposure to atmospheric oxygen, the environmental niches most frequently occupied. R postgategrowth of sulfatereducing bacteria in sulfatefree media. Desulfovibrio species are commonly found in aquatic environments with high levels of organic material, as well as in waterlogged soils, and form major community members of extreme oligotrophic habitats such as deep granitic fractured rock aquifers like other sulfatereducing bacteria, desulfovibrio was long considered to. It is generally found in soils, waters, and the stools of animals, although in rare cases it has been found to cause infection in humans. To explore the physiological role of tetraheme cytochrome c 3 in the sulfatereducing bacterium desulfovibrio desulfuricans g20, the gene encoding the preapoprotein was cloned, sequenced, and mutated by plasmid insertion. The properties of the periplasmic hydrogenase from desulfovibrio desulfuricans atcc 7757, previously reported to be a singlesubunit protein glick, b. Desulfovibrio cells have mesophillic growth patterns with an ideal growth rate between the. The rcsb pdb also provides a variety of tools and resources.
Desulfovibrio gigas carried two plasmids of 70 mdal 105. Ftir characterization of the active site of the fe. Sulfatereducing bacterium desulfovibrio desulfuricans nd2 as. Desulfovibrio desulfuricans beijerinck 1895 kluyver and van niel 1936, 397 spirillum desulfuricans beijerinck 1895, 1.
Further characterization of the fehydrogenase from. A study of spore formation and other morphological characteristics of vibrio desulfuricans. Dde i from desulfovibrio desulfuricans, strain norway. It was chosen for study because of its exceptionally high rates of hg methylation in culture and its metabolic. Protein science aims to unify this field by cutting across.
The crystal structure of desulfovibrio desulfuricans napa reveals that the. A case of liver abscess with desulfovibrio desulfuricans. Nitrate reduction was induced by nitrate or limiting concentrations of sulfate during growth, while all other activities were constitutive. Desulfovibrio strains have been found in a variety of habitats, including soil, the intestines and feces of animals, and both salinic and fresh water. The strains benghazi and walvis bay can be distinguished from 40 strains of desulfovibrio and from d. Desulfovibrio vulgaris postgate and campbell atcc 7757. Several groups of researchers have reported desulfovibrio like organisms, based on 16s rdna sequence similarity, associated with proliferative bowel diseases from ferrets. Desulfovibrio desulfuricans bacteremia in an immunocompromised. Several studies have shown that dom can enhance hg methylation, especially under sulfidic conditions, whereas others show that dom inhibits hg methylation due to strong hgdom complexation. Strain nd2 is an anaerobic dissimilatory sulfatereducing bacterium dsrb, isolated from estuarine midchesapeake bay sediments.
At only 6 m, cuii reduced the maximum specific growth rate by 25% and the final cell protein concentration by 18% compared to the copperfree control. It is particularly noted for its ability to produce methyl mercury. Desulfovibrio desulfuricans strain nd 2 was isolated from chesapeake bay sediments in may 1985 gilmour et al. Desulfovibrio desulfuricans aestuarii postgate and campbell, 1966 subspecies. Purification and partial characterization of a dlactate dehydrogenase from desulfovibrio desulfuricans atcc 7757. The concentrations of cellsurface and intracellular thiols were measured, and 9 specific fractions of d. Pmc free article czjzek m, payan f, guerlesquin f, bruschi m, haser r. The genome sequence of the anaerobic, sulfatereducing. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
Sequencing of sulfonatemetabolizing anaerobes is key for understanding sulfonate degradation and its role in the sulfur cycle. Effect of a rosmarinic acid supplemented hemodialysis fluid on inflammation of human vascular endothelial cells. Bonaventure, newyork14778 receivedfor publication 26april 1968 thelalanine dehydrogenase fromcell free extracts ofdesulfovibrio desulfuricans was purified approximately 56fold. Dec 25, 2001 desulfovibrio desulfuricans wild type strain essex 6 atcc 29577 was used in this study and cultured in lactate. The journal publishes papers by leading scientists from all over the world that report on advances in the understanding of proteins in the broadest sense. While most isolates have not been identified to the species level, desulfovibrio fairfieldensis and d.
Pronunciation of desulfovibrio with 1 audio pronunciation, 1 translation and more for desulfovibrio. Desulfovibrio and desulfuromonas reduce sulfate and elemental sulfur s, respectively, yielding sulfide s2. Desulfovibrio vulgaris hildenborough is a model organism for studying the energy metabolism of sulfatereducing bacteria srb and for understanding the economic impacts of. Its cells are gramnegative, slightly curved rods gilmour et al. As a member of the wwpdb, the rcsb pdb curates and annotates pdb data according to agreed upon standards.
Desulfovibrio is a genus of gramnegative sulfatereducing bacteria. Sulfatereducing bacterium desulfovibrio desulfuricans. Desulfovibrio cells have mesophillic growth patterns with an ideal growth rate between the temperatures of twentyfive to fourty degrees celcius ncbi. Pdf sulfatereducing bacterium desulfovibrio desulfuricans.
We propose the use of desulfovibrio desulfuricans nd2 as a model species for understanding the mechanism of microbial hg methylation. Desulfovibrio species are commonly found in aquatic environments with high levels of organic material, as well as in waterlogged soils, and form major community members of extreme oligotrophic habitats such as deep granitic fractured rock aquifers. We propose the use of desulfovibrio desulfuricans nd2 as a model species for. Desulfovibrio desulfuricans applied and environmental. Desulfovibrio desulfuricans has been reported to be the cause of a case of gastrointestinal disorder and bacteremia with episodes of fever, chills, and profuse perspiration. Johns hospital health center, santa monica, california 90404 3. Desulfovibrio desulfuricans strain atcc 27774 dsm 6949 is a sulfatereducing bacterium isolated from the rumen of a sheep. An antibioticproducing, obligate anaerobic, gramstainnegative, catalase and oxidasenegative strain jc271t was isolated from a marine habitat and identified, based on 16s rrna gene sequence analysis, as a novel member of the family desulfovibrionaceae. We report the complete genome sequence of the anaerobic, sulfonaterespiring, sulfatereducing bacterium desulfovibrio desulfuricans ic1. Journal of materials engineering and performance 20, 22 2. Comparison of biocorrosion due to desulfovibrio desulfuricans and desulfotomaculum nigrificans bacteria. In this study, the antimicrobial activity and corrosion inhibition effect of a lowtoxic. Degradation of nitrocellulosebased paint by desulfovibrio desulfuricans atcc 541 l.
Degradation of nitrocellulosebased paint by desulfovibrio. Desulfovibrio desulfuricans desulfuricans beijerinck, 1895 kluyver and van niel, 1936. Cytochrome c 3 mutants ofdesulfovibrio desulfuricans. The main source of no for freeliving bacteria is nitrite generated either as a product of their own metabolism, or by other bacteria that share their. Complete genome sequence of desulfovibrio desulfuricans ic1. The physical analysis of the dna from the strain carrying the integrated plasmid showed that the insertion was successful. Desulfovibrio article about desulfovibrio by the free. Desulfovibrio desulfuricans bacteremia in a dog journal of. Hydrogen metabolism in desulfovibrio desulfuricans strain new jersey ncimb 83 comparative. Metabolic pathways leading to mercury methylation in desulfovibrio desulfuricans ls. The desulfovibrio desulfuricans formate dehydrogenase has been clearly demonstrated to catalyze co 2 reduction using a viologen as reductant, and the reaction has recently been comprehensively examined, with k cat in the reverse direction being determined to be 47 s.
Dna preparation, polymerase chain reaction pcr, dna sequencing and computational analysis were all carried out as previously described morse et al. Journal of materials engineering and performance 20, 22 2, 463469. Desulfovibrio desulfuricans beijerinck, 1895 kluyver and van niel, 1936. Complete genome sequence of desulfovibrio desulfuricans. Direct spectroscopic evidence for the presence of a 6fe cluster in an ironsulfur protein isolated from desulfovibrio desulfuricans atcc 27774 published erratum appears in j. The reported case of a desulfovibrio desulfuricans. Contrasting effects of dissolved organic matter on mercury. Effects of oxygen on the growth of desulfovibrio desulfuricans free. The toxicity of al to desulfovibrio desulfuricans g20 was assessed over a period of 8 weeks in a modi. Desulfovibrio desulfuricans an overview sciencedirect topics.
Analyses of the amino acid sequences indicated a number of serohaemfe 4 s 4 binding consensus sequences whilst predictive secondary structure analysis revealed a similar pattern of helix and strand. Alden research laboratory, 1 infectious diseases division, 2 microbiology laboratory, st. The authors have examined extracellular ironbearing precipitates resulting from the growth of desulfovibrio desulfuricans in a basal medium with lactate as the carbon source and ferrous sulfate. Effects of oxygen exposure on respiratory activities of. The closest phylogenetic relatives of strain jc271t were found to be desulfovibrio marinisediminis cl2t 99. Desulfuricans nadhoxidation between the complete system and the nosubstrate control. Regulation, sensory domains and roles of two desulfovibrio. Single plasmids were also detected in a second strain of d. At ph 5, cell population densities decreased signi. Mehg production was also measured in cellfree spent medium by adding. R smithbergeys manual of determinative bacteriology. Effects of oxygen on the growth of desulfovibrio desulfuricans. Presence of the cofactor speeds up folding of desulfovibrio. Desulfovibrio desulfuricans is a gramnegative sulfatereducing bacteria.
Protein science, the flagship journal of the protein society, serves an international forum for publishing original reports on all scientific aspects of protein molecules. The sulfatereducing bacterium desulfovibrio desulfuricans strain csn dsm 104 oxidized h 2 with thiosulfate, sulfate, sulfite, nitrite, nitrate and oxygen with rates increasing in the order listed from 20 to 525 nmol h 2 min. Sulfatereducing bacterium desulfovibrio desulfuricans nd2. Desulfovibrio desulfuricans strain atcc 27774 dsm 6949. It was chosen for study because of its exceptionally high rates of hg methylation in culture and its metabolic similarity to the. Desulfovibrio desulfuricans bacteremia in a dog journal. This organism grows anaerobically and utilizes a wide variety of electron acceptors, including.
Desulfovibrio desulfuricans an overview sciencedirect. The increasing restrictions of environmental regulations and growing safety concerns on the use of biocides result in efforts to minimize the amount of biocide use and develop environmentally friendly biocides. Thepartially purified enzymewasstablefor26hr at 0c. Anaerobic oxidation of hydrocarbons by desulfovibrio desulfuricans. Natural dissolved organic matter dom affects mercury hg redox reactions and anaerobic microbial methylation in the environment. Sulfatereducing bacterium desulfovibrio desulfuricans nd2 as a model for understanding bacterial mercury methylation article pdf available in applied and environmental microbiology 7712. In this study, we investigated and compared the effects of dom on hg. Identification of these species with conventional methods is difficult. Secondary structure analysis of the dissimilatory sulphite. Desulfovibrio desulfuricans lipopolysaccharides induce endothelial cell il6 and il8 secretion and eselectin and vcam1 expression. Strain details desulfovibrio desulfuricans strain g20 is a spontaneously nalidixic acid resistant derivative of the wildtype strain g100a that was isolated from an oil well corrosion site weimer et al. Nolactic dehydrogenase activity was ob served with the partially purified preparation. Desulfovibrio desulfuricans also known as strain nd 2 description and significance.
Crystal structure of cytochrome c3 from desulfovibrio desulfuricans norway at 1. In introducing his work, beyerinck pointed out that the microbial formation of hydrogen sulfide is of great importance and interest from both purely scientific and applied points of view. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. Pdf we propose the use of desulfovibrio desulfuricans nd2 as a model species for. Pdf hydrogen metabolism in desulfovibrio desulfuricans strain. Further characterization of the fejhydrogenase from desulfovibrio desulfuricans atcc 7757 e. Characterization of palladium nanoparticles produced by healthy and microwaveinjured cells of desulfovibrio desulfuricans and escherichia coli by jaime gomezbolivar 1, iryna p.